Curation Information

Publication
PhhR binds to target sequences at different distances with respect to RNA polymerase in order to activate transcription.;Herrera MC, Krell T, Zhang X, Ramos JL;Journal of molecular biology 2009 Dec 4; 394(3):576-86 [19781550]
TF
PhhR [Q88EH4, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Kathryn Cronise
Curation notes
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Experimental Process

A PSSM search was performed with known phhA sites and the consensus for PhhR. Isothermal titration calorimetry (ITC) was used to show that binding is favorable.

Transcription Factor Binding Sites


CACAAGTGATACACGATTGACGACCA
CACAAGTGATACACGATTGACGACCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CACAAGTGATACACGATTGACGACCA
... ... PP_3284 phaB paaC PP_3281 phaD phaM
Experimental technique details Isothermal titration calorimetry (ECO:0005647) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer