Curation Information

Fur activates expression of the 2-oxoglutarate oxidoreductase genes (oorDABC) in Helicobacter pylori.;Gilbreath JJ, West AL, Pich OQ, Carpenter BM, Michel S, Merrell DS;Journal of bacteriology 2012 Dec; 194(23):6490-7 [23002221]
Fur [B5Z6G7, view regulon]
Reported TF sp.
Helicobacter pylori G27
Reported site sp.
Helicobacter pylori G27
Created by
Dinara Sagitova
Curation notes

Experimental Process

qRT-PCR and primer extension techniques were applied to show Fur-mediated activation of transcription. Specifically, the expression level of the target genes was decreased in a fur mutant strain. Binding of Fur to the putative binding site in the oorDABC promoter region was confirmed by fluorescence anisotropy assays.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... oorD HPG27_547 HPG27_546 HPG27_545 HPG27_544 oorA oorB oorC
Experimental technique details Fluorescence anisotropy (ECO:0005632) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified