Curation Information

Publication
Transcriptional regulation of Pseudomonas aeruginosa rhlR: role of the CRP orthologue Vfr (virulence factor regulator) and quorum-sensing regulators LasR and RhlR.;Croda-García G, Grosso-Becerra V, Gonzalez-Valdez A, Servín-González L, Soberón-Chávez G;Microbiology (Reading, England) 2011 Sep; 157(Pt 9):2545-55 [21719541]
TF
Vfr [P55222, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Kathryn Cronise
Curation notes
-

Experimental Process

A beta-gal assay was performed to show that Vfr activates rhlR expression. A consensus search identified putative binding sites. Each site was then knocked-out and a beta-gal assay was performed to see the effect. Three binding sites involved in rhlR activation were identified.

Transcription Factor Binding Sites


AAGGCTGCGCGCTTGACAG
TCCTGAACGGTGCTGGCAT
TGGTCGAACGTTGTCATAG
AAGGCTGCGCGCTTGACAG
TCCTGAACGGTGCTGGCAT
TGGTCGAACGTTGTCATAG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AAGGCTGCGCGCTTGACAG rhlR,
... ... rhlR rhlB
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - activator not specified
TCCTGAACGGTGCTGGCAT rhlR,
... ... rhlR rhlB rhlA
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - activator not specified
TGGTCGAACGTTGTCATAG rhlR,
... ... rhlR rhlB rhlA
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - activator not specified