Curation Information

Publication
Analysis of LuxR regulon gene expression during quorum sensing in Vibrio fischeri.;Qin N, Callahan SM, Dunlap PV, Stevens AM;Journal of bacteriology 2007 Jun; 189(11):4127-34 [17400743]
TF
LuxR [B5EV73, view regulon]
Reported TF sp.
Vibrio Fischeri MJ-1
Reported site sp.
Vibrio Fischeri MJ-1
Created by
Elliot White
Curation notes
-

Experimental Process

Researchers used LacZ fusions (in E. Coli) to determine the regulatory impact of LuxR on the putative lux boxes. They then ran EMSA's with those that showed a change in regulation.

Transcription Factor Binding Sites


ACCTGTAGGATCGTACAGGT
ACCTGTAGGATCGTACAGGT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ACCTGTAGGATCGTACAGGT VFMJ11_A1042,
... ... VFMJ11_A1042 VFMJ11_A1043 VFMJ11_A1044
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - activator dimer