Linear DNA carrying the resistance cassette and flanking regions was generated by PCR.β-galactosidase assays approached to measure the B-galactosidase activity after overnight incubation of the broth containing appropriate antibiotics in anaerobic conditions at 37 °C.site-directed mutagenesis of the FNR site was achieved using PCR and appropriate synthetic oligonucleotides. Footprinting reactions with FNR were done essentially with PydhY used as the target DNA
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|