Curation Information

Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP.;Partridge JD, Browning DF, Xu M, Newnham LJ, Scott C, Roberts RE, Poole RK, Green J;Microbiology (Reading, England) 2008 Feb; 154(Pt 2):608-18 [18227264]
FNR [P0A9E5, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Sepideh Zahiri
Curation notes

Experimental Process

Linear DNA carrying the resistance cassette and flanking regions was generated by PCR.β-galactosidase assays approached to measure the B-galactosidase activity after overnight incubation of the broth containing appropriate antibiotics in anaerobic conditions at 37 ° mutagenesis of the FNR site was achieved using PCR and appropriate synthetic oligonucleotides. Footprinting reactions with FNR were done essentially with PydhY used as the target DNA

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ydhY ydhV ydhW ydhZ
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - activator dimer