Curation Information

Publication
Global analysis of the regulon of the transcriptional repressor LexA, a key component of SOS response in Mycobacterium tuberculosis.;Smollett KL, Smith KM, Kahramanoglou C, Arnvig KB, Buxton RS, Davis EO;The Journal of biological chemistry 2012 Jun 22; 287(26):22004-14 [22528497]
TF
LexA [I6Y1J7, view regulon]
Reported TF sp.
Mycobacterium tuberculosis H37Rv
Reported site sp.
Mycobacterium tuberculosis H37Rv
Created by
Erill Lab
Curation notes
-

Experimental Process

ChIP-Seq plus ChIP-PCR to validate binding Expression data assessed with beta-gal assays and RT-PCR

ChIP assay conditions
MtuLexA: The mycobacterial strains used were wild-type M. tuberculosis strains H37Rv and 1424 and M. tuberculosis
ChIP notes
Chromatin Immunoprecipitation (ChIP)—ChIP was performed using a method adapted from Sala et al.(16). Mycobacterial cultures were grown to mid-log phase (A600

Transcription Factor Binding Sites


TCGAACAATTGTTCGATA
TCGAACGTATGTTCGAGA
TCGAACACATGTTCGAGA
TCGAACAATTGTTCGATA
TCGAACGTATGTTCGAGA
TCGAACACATGTTCGAGA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCGAACAATTGTTCGATA
... ... RVBD_3370c RVBD_3371 RVBD_3372
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - repressor dimer
TCGAACGTATGTTCGAGA
... ... RVBD_3778c RVBD_3777 RVBD_6095 RVBD_3772 RVBD_3773c RVBD_3774 RVBD_3775
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - repressor dimer
TCGAACACATGTTCGAGA RVBD_3074,
... ... RVBD_3074 RVBD_3075c RVBD_3072c RVBD_3073c
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - repressor dimer