Curation Information

Publication

Transcriptional activation by FNR and CRP: reciprocity of binding-site recognition.;Sawers G, Kaiser M, Sirko A, Freundlich M;Molecular microbiology 1997 Feb; 23(4):835-45 [9157253]

TF

CRP [P0ACJ8, view regulon]

Reported TF sp.

Escherichia coli BW2952

Reported site sp.

mutagenesis-induced

Created by

Patrick O'Neill

Curation notes

-

Experimental Process

Construction of mutant sites by site-directed mutagenesis Demonstration of binding by DNAse footprinting. Demonstration of regulation by primer extension and beta-gal reporter assay.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.