Curation Information

Publication
The global virulence regulator PhcA negatively controls the Ralstonia solanacearum hrp regulatory cascade by repressing expression of the PrhIR signaling proteins.;Yoshimochi T, Hikichi Y, Kiba A, Ohnishi K;Journal of bacteriology 2009 May; 191(10):3424-8 [19060134]
TF
PhcA [P52698, view regulon]
Reported TF sp.
Ralstonia solanacearum OE1-1
Reported site sp.
Ralstonia solanacearum OE1-1
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding of PhcA to the xpsR and prhIR promoters was demonstrated with EMSA. The specific binding site in the prhIR promoter was determined with EMSA using varied-length fragments of the promoter. Using a phcA deletion mutant, beta-gal assays with varied-length fragments of the prhIR promoter were assessed for expression. The putative binding site of PhcA in prhIR was determined through visual inspection of the fragments that bound PhcA and that deregulated expression. The putative binding site for the xpsR promoter had been reported previously (PMID: 7868600).

Transcription Factor Binding Sites


ATGAGGTTCACGCAT
TTAAAAAATCTTTAC
ATGAGGTTCACGCAT
TTAAAAAATCTTGAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATGAGGTTCACGCAT
... ... RS_RS21185 RS_RS21190 RS_RS21195
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified
TTAAAAAATCTTGAT RS_RS21960,
... ... RS_RS21960 RS_RS21955 RS_RS21950 RS_RS21965
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified