Curation Information

Publication
A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW.;Modell JW, Hopkins AC, Laub MT;Genes & development 2011 Jun 15; 25(12):1328-43 [21685367]
TF
LexA [Q9A724, view regulon]
Reported TF sp.
Caulobacter crescentus CB15
Reported site sp.
Caulobacter crescentus CB15
Created by
Erill Lab
Curation notes
-

Experimental Process

SidA (CC1927) was identified as a likely LexA-regulated gene based on the previously reported presence of a putative LexA-binding site in its promoter region (through PSSM-search) and its rapid induction in response to mitomycin and UV light. Its role as an SOS induced cell-division inhibitor was demonstrated by the loss of filamentation in a double sidA-lexA mutant.

Transcription Factor Binding Sites


CATATTCCGGTTTTGTTCT
AGATTTCTTGTTTTGTTCC
TCTGTTCACAAGATGTTCC
CATATTCCGGTTTTGTTCT
AGATTTCTTGTTTTGTTCC
TCTGTTCACAAGATGTTCC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CATATTCCGGTTTTGTTCT CC_1927,
... ... CC_1927 dnaE CC_1928
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified
AGATTTCTTGTTTTGTTCC CC_1927,
... ... CC_1927 dnaE CC_1928
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified
TCTGTTCACAAGATGTTCC CC_1927,
... ... CC_1927 dnaE CC_1928
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified