Curation Information

Helicobacter pylori HP1512 is a nickel-responsive NikR-regulated outer membrane protein.;Davis GS, Flannery EL, Mobley HL;Infection and immunity 2006 Dec; 74(12):6811-20 [17030579]
NikR [O25896, view regulon]
Reported TF sp.
Helicobacter pylori 26695
Reported site sp.
Helicobacter pylori 26695
Created by
Matthew Coveyou
Curation notes

Experimental Process

The Helicobacter pylori HP1512 gene was identified as a gene of interest using a whole-genome microarray. Quantitative PCR measured transcription of HP1512 in nickel-variable cultures, and a nickel supplement of 10 uM resulted in a 43-fold decrease in transcription. A consensus search identified a putative binding site that was a perfect match to the operator consensus sequence, and EMSA demonstrated site-specific binding of NikR to the target site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... HP1512
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - repressor tetramer