Curation Information

Regulation of the Escherichia coli lrp gene.;Wang Q, Wu J, Friedberg D, Plakto J, Calvo JM;Journal of bacteriology 1994 Apr; 176(7):1831-9 [8144448]
Lrp [P0ACJ0, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Erill Lab
Curation notes

Experimental Process

The promoter of lrp was mapped with primer extension. Regulation by Lrp was shown by plrp-lacZ reporter assays comparing w-t and lrp mutant, using truncations and site-directed mutagenesis of the promoter region containing putative sites. Binding was shown first with EMSA on truncated plrp fragments and then confirmed with DNAse footprinting.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... lrp ftsK aat cydC cydD trxB
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer