The TSS was identified by primer extension. VpsR sites in the VpsL promoter were identified by DNAse footprinting and their specificity validated by EMSA with mutated sites and Lux reporter assays with same site mutants and promoters truncated at several distances from the TSS. The motif was inferred by motif discovery on biofilm formation-related promoters and its stability validated by comparative genomics on V. cholerae isolates.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|