Curation Information

Publication
Global regulation of gene expression by OxyR in an important human opportunistic pathogen.;Wei Q, Minh PN, Dötsch A, Hildebrand F, Panmanee W, Elfarash A, Schulz S, Plaisance S, Charlier D, Hassett D, Häussler S, Cornelis P;Nucleic acids research 2012 May; 40(10):4320-33 [22275523]
TF
OxyR [Q9HTL4, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

ChIP-chip analysis identified 122 genes (enrichment >0.5) belonging to the OxyR regulon in P.aeruginosa. ChIP-chip results for selected genes were validated with EMSA for in vitro binding and qRT-PCR for gene expression. Virtual Footprint program was used to search P. aeruginosa PAO1 genome for OxyR-like binding sequences. The authors report that eighty-eight out of the ChIPchip identified 122 genes (72.13%, cutoff > 0.5, Log2 ratio) or 42 out of 57 genes (73.68%, cutoff > 0.7, Log2 ratio) were identified as having a consensus motif-like sequence in the corresponding promoter region.

ChIP assay conditions
Bacteria were grown in Luria-Bertani (L.B.) broth until early stationary phase (OD600 = 2.0) and 1 mM H2O2 was added to treat the cultures for 10 min. Formaldehyde was then added to a final concentration of 1%. After incubation for 20 min at room temperature, glycine was added to a final concentration of 0.5 M. Cells were harvested by centrifugation, washed twice in ice-cold Tris-buffered saline (TBS, pH 7.6) and resuspended in 500 µl of lysis buffer [10 mM Tris–HCl, pH 8.0, 20% sucrose, 50 mM NaCl, 10 mM ethylenediaminetetraacetic acid (EDTA)]. Lysozyme (2.5 mg) was added and the cells were incubated for 30 min at 37°C. The DNA was sheared to an average size of 100–1000 bp by a series of pulsed sonication steps (Branson Sonicator S250 Analogue, 5 mm Standard tip, six times 60 s at 90% duty on Level 4).
ChIP notes
Insoluble material was removed by centrifugation at 13 000g for 10 min and 850 µl of the cell extract supernatant from His-tagged or non-His-tagged control bacterial strain were mixed with 750 µl of immunoprecipitation (IP) buffer [50 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (m/v) sodium deoxycholate] and phenylmethanesulfonyl fluoride (PMSF, final concentration 1 mM) and incubated with 20 µl of protein G-Sepharose beads (Sigma) for 1 h at room temperature with gentle mixing. After removal of the Sepharose beads by gentle centrifugation, proteins were immunoprecipitated by adding 20 µl of protein G-Sepharose beads (Sigma) coupled to mouse anti-His monoclonal antibody (Novagen). The composite was incubated overnight at 4°C with gentle mixing. Samples were then washed twice with IP buffer, once with IP buffer containing 500 mM NaCl, once with washing buffer [10 mM Tris–HCl, pH 8.0, 250 mM LiCl, 1 mM EDTA, 0.5% (v/v) Nonidet-P40, 0.5% (m/v) sodium deoxycholate] and once with TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7.5). Immunoprecipitated complexes were eluted by incubation of the beads with elution buffer [50 mM Tris–HCl, pH 7.5, 10 mM EDTA, 1% (m/v) sodium dodecyl sulfate (SDS)] at 65°C for 10 min. Forty microliters of the immunoprecipitated samples were de-cross-linked by the addition of 32 µg Pronase and incubation for 2 h at 42°C and for 6 h at 65°C. DNA was purified using polymerase chain reaction (PCR) Purification kit (Qiagen, Germany). All ChIPs were performed in duplicate.

Transcription Factor Binding Sites


ATAGATTTAGATAAT
ATAGGCTGACTCTAT
ATTGATCTCGCTTAT
ATTGATATTCCTAAT
ATTGGTATTGCTAAT
ATAGGGAGAATCTAT
AAAGCTGGAAACAAT
ATCTTTGAACCCTAT
ATGAGCCTGGGTTAT
ATCGAAGCGCTCTAT
ATAGAGACTCTCAAT
ATAATTATTGATTTT
ATAAATTCAGATTTT
ATATTTACCGGTTAT
ATGGCGGCTAGCTAT
AAAAAGAATATTAAT
ATTGGTCTGGTCTAT
ATGGTTTGCAATTAT
ATGGTTTGCAATTAT
AATTTTTTAATCTAT
ATTGACAATCATTAT
AAGTGTAGCGCCTAT
ATAGAACTGGTCTAT
ATAGGTTTATATGAT
ATAATTATTGATTTT
ATAGAGCAAGACTAT
ATTTCTAACAACGAT
AACGGTTTTTATAAT
ATATTTCCGACGAAT
ATCGCTATCGCCGAT
ATAGAGACTCTCAAT
ATTTTTATTATTTAT
ATTTTTATTATTTAT
ATTAAAAACATTAAT
ATAGATGCGGCCAAT
ATAGTTATTGCCTAT
ATAGGGAGAATCTAT
ATATTTACCGGTTAT
ATAGATTGCGATAAT
ATAACGTCGGGTTAT
ATCGATTTTTTGTAT
ATCGATTGGCTTTTT
ATTGGTCTGGTCTAT
ATTGATATTTGCAAT
ATTGAAGCACTTAAT
ATAGACTCAGGCTAT
AAGTGTAGCGCCTAT
ATCGAAGCGCTCTAT
ATAAGTTCGGTGAAT
AATGAGAAAATCTAT
ATATAAAAACGCTAT
ATAGCTCCACTGATT
ACTGTTTTCAACAAT
ATCTCTATAATGAAT
ATTTCTTTTTACGAT
ATAGAACTGGTCTAT
ATAGGTTTATATGAT
ATCGTTGATGCCGAT
ATCGTGATAGCTAAT
ATTGTTCTCTTTATT
ATAGGAAAAGTTGAT
ATAGGAAAAGTTGAT
AATTATTTTTGTTAT
ATAGATGCGGCCAAT
ATAAACTACGTAAAT
ATTGCCGCAGCCAAT
ATCATCGGTAGTTAT
AGAGGCGGAGGCAAT
ATTTGCCTTCTCGAT
ATGGCTTTATATAAT
ATATTTCCGACGAAT
AGCTCTTCAGACTAT
ATTGATAAAACCTAT
ATAGACCCATTCAAT
ATTGATATTTGCAAT
ATAGATTCAGTCTTT
ATAGAGAGTACTAAT
ATAGATTATTTGAAT
ATCGATCTGATCGAT
ATCAAACGTATTTAT
ATCAAACGTATTTAT
ATGGCTTTTTGCTAT
ATAAAAAACATTTAT
ATCTCTATAATGAAT
ATCGACGTGATCTAT
TTTGAAAAAAACTAT
ATAGCAAAACTCTAT
ATAGCAAAACTCTAT
ATAGATTTAGATAAT
ATAGGCTGACTCTAT
ATTGATCTCGCTTAT
ATTGATATTCCTAAT
ATTGGTATTGCTAAT
ATAGGGAGAATCTAT
AAAGCTGGAAACAAT
ATCTTTGAACCCTAT
ATGAGCCTGGGTTAT
ATCGAAGCGCTCTAT
ATAGAGACTCTCAAT
ATAATTATTGATTTT
ATAAATTCAGATTTT
ATATTTACCGGTTAT
ATGGCGGCTAGCTAT
AAAAAGAATATTAAT
ATTGGTCTGGTCTAT
ATGGTTTGCAATTAT
ATGGTTTGCAATTAT
AATTTTTTAATCTAT
ATTGACAATCATTAT
AAGTGTAGCGCCTAT
ATAGAACTGGTCTAT
ATAGGTTTATATGAT
ATAATTATTGATTTT
ATAGAGCAAGACTAT
ATTTCTAACAACGAT
AACGGTTTTTATAAT
ATATTTCCGACGAAT
ATCGCTATCGCCGAT
ATAGAGACTCTCAAT
ATTTTTATTATTTAT
ATTTTTATTATTTAT
ATTAAAAACATTAAT
ATAGATGCGGCCAAT
ATAGTTATTGCCTAT
ATAGGGAGAATCTAT
ATATTTACCGGTTAT
ATAGATTGCGATAAT
ATAACGTCGGGTTAT
ATCGATTTTTTGTAT
ATCGATTGGCTTTTT
ATTGGTCTGGTCTAT
ATTGATATTTGCAAT
ATTGAAGCACTTAAT
ATAGACTCAGGCTAT
AAGTGTAGCGCCTAT
ATCGAAGCGCTCTAT
ATAAGTTCGGTGAAT
AATGAGAAAATCTAT
ATATAAAAACGCTAT
ATAGCTCCACTGATT
ACTGTTTTCAACAAT
ATCTCTATAATGAAT
ATTTCTTTTTACGAT
ATAGAACTGGTCTAT
ATAGGTTTATATGAT
ATCGTTGATGCCGAT
ATCGTGATAGCTAAT
ATTGTTCTCTTTATT
ATAGGAAAAGTTGAT
ATAGGAAAAGTTGAT
AATTATTTTTGTTAT
ATAGATGCGGCCAAT
ATAAACTACGTAAAT
ATTGCCGCAGCCAAT
ATCATCGGTAGTTAT
AGAGGCGGAGGCAAT
ATTTGCCTTCTCGAT
ATGGCTTTATATAAT
ATATTTCCGACGAAT
AGCTCTTCAGACTAT
ATTGATAAAACCTAT
ATAGACCCATTCAAT
ATTGATATTTGCAAT
ATAGATTCAGTCTTT
ATAGAGAGTACTAAT
ATAGATTATTTGAAT
ATCGATCTGATCGAT
ATCAAACGTATTTAT
ATCAAACGTATTTAT
ATGGCTTTTTGCTAT
ATAAAAAACATTTAT
ATCTCTATAATGAAT
ATCGACGTGATCTAT
TTTGAAAAAAACTAT
ATAGCAAAACTCTAT
ATAGCAAAACTCTAT

Quantitative data format: Enrichment factor

ATAGATTTAGATAAT 1.72
ATAGGCTGACTCTAT 0.83
ATTGATCTCGCTTAT 1.61
ATTGATATTCCTAAT 0.53
ATTGGTATTGCTAAT 0.63
ATAGGGAGAATCTAT 1.02
AAAGCTGGAAACAAT 0.64
ATCTTTGAACCCTAT 1.04
ATGAGCCTGGGTTAT 0.55
ATCGAAGCGCTCTAT 0.65
ATAGAGACTCTCAAT 0.78
ATAATTATTGATTTT 0.87
ATAAATTCAGATTTT 0.52
ATATTTACCGGTTAT 0.74
ATGGCGGCTAGCTAT 0.54
AAAAAGAATATTAAT 1.27
ATTGGTCTGGTCTAT 1.07
ATGGTTTGCAATTAT 0.61
ATGGTTTGCAATTAT 0.61
AATTTTTTAATCTAT 0.54
ATTGACAATCATTAT 0.71
AAGTGTAGCGCCTAT 1.22
ATAGAACTGGTCTAT 0.66
ATAGGTTTATATGAT 0.64
ATAATTATTGATTTT 0.87
ATAGAGCAAGACTAT 1.23
ATTTCTAACAACGAT 0.52
AACGGTTTTTATAAT 0.57
ATATTTCCGACGAAT 0.77
ATCGCTATCGCCGAT 0.71
ATAGAGACTCTCAAT 0.78
ATTTTTATTATTTAT 0.83
ATTTTTATTATTTAT 0.83
ATTAAAAACATTAAT 1.17
ATAGATGCGGCCAAT 0.59
ATAGTTATTGCCTAT 1.42
ATAGGGAGAATCTAT 1.02
ATATTTACCGGTTAT 0.74
ATAGATTGCGATAAT 1.48
ATAACGTCGGGTTAT 0.68
ATCGATTTTTTGTAT 0.54
ATCGATTGGCTTTTT 1.2
ATTGGTCTGGTCTAT 1.07
ATTGATATTTGCAAT 0.64
ATTGAAGCACTTAAT 0.65
ATAGACTCAGGCTAT 1.34
AAGTGTAGCGCCTAT 1.22
ATCGAAGCGCTCTAT 0.65
ATAAGTTCGGTGAAT 0.55
AATGAGAAAATCTAT 0.62
ATATAAAAACGCTAT 0.6
ATAGCTCCACTGATT 1.23
ACTGTTTTCAACAAT 1.27
ATCTCTATAATGAAT 0.59
ATTTCTTTTTACGAT 0.53
ATAGAACTGGTCTAT 0.66
ATAGGTTTATATGAT 0.64
ATCGTTGATGCCGAT 0.6
ATCGTGATAGCTAAT 1.36
ATTGTTCTCTTTATT 0.53
ATAGGAAAAGTTGAT 0.69
ATAGGAAAAGTTGAT 0.69
AATTATTTTTGTTAT 0.56
ATAGATGCGGCCAAT 0.59
ATAAACTACGTAAAT 0.83
ATTGCCGCAGCCAAT 0.53
ATCATCGGTAGTTAT 1.27
AGAGGCGGAGGCAAT 0.52
ATTTGCCTTCTCGAT 0.67
ATGGCTTTATATAAT 0.58
ATATTTCCGACGAAT 0.77
AGCTCTTCAGACTAT 0.86
ATTGATAAAACCTAT 1.25
ATAGACCCATTCAAT 1.04
ATTGATATTTGCAAT 0.64
ATAGATTCAGTCTTT 0.72
ATAGAGAGTACTAAT 0.72
ATAGATTATTTGAAT 0.58
ATCGATCTGATCGAT 0.7
ATCAAACGTATTTAT 0.56
ATCAAACGTATTTAT 0.56
ATGGCTTTTTGCTAT 0.72
ATAAAAAACATTTAT 0.59
ATCTCTATAATGAAT 0.59
ATCGACGTGATCTAT 0.61
TTTGAAAAAAACTAT 0.52
ATAGCAAAACTCTAT 0.79
ATAGCAAAACTCTAT 0.79

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATAGATTTAGATAAT ahpC,
... ... ahpC ahpF
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - not specified not specified
ATAGGCTGACTCTAT PA0848,
... ... PA0848 PA0847 PA0846 trxB2
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGATCTCGCTTAT katA,
... ... katA bfrA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGATATTCCTAAT katB,
... ... katB PA4612 PA4611 mscL
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGGTATTGCTAAT oprE
... ... oprE
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGGAGAATCTAT PA0962,
... ... PA0962 aspS pmpR ruvC ruvA ruvB PA0968 tolQ tolR tolA tolB oprL PA0974 PA0975 PA0976 PA0976.1
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
AAAGCTGGAAACAAT PA1289
... ... PA1289
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCTTTGAACCCTAT PA2146,
... ... PA2146 PA2145 katE
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGAGCCTGGGTTAT PA2821
... ... PA2821
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGAAGCGCTCTAT PA3237,
... ... PA3237 PA3236 PA3238 PA3239 PA3240
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAGACTCTCAAT trxA,
... ... trxA ppx
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ATAATTATTGATTTT PA5436,
... ... PA5436 PA5435 PA5437
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAAATTCAGATTTT PA3450,
... ... PA3450 PA3451
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATATTTACCGGTTAT mvfR,
... ... mvfR nadA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGGCGGCTAGCTAT PA1159
... ... PA1159
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
AAAAAGAATATTAAT PA2050,
... ... PA2050 PA2049 PA2051
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGGTCTGGTCTAT cynR,
... ... cynR cynT cynS
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGGTTTGCAATTAT ptxR,
... ... ptxR ptxS PA2260 PA2261 PA2262 PA2263
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGGTTTGCAATTAT ptxS,
... ... ptxS ptxR PA2260 PA2261 PA2262 PA2263
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
AATTTTTTAATCTAT PA2359,
... ... PA2359 PA2358
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGACAATCATTAT pvdS,
... ... pvdS pvdG pvdL
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - not specified not specified
AAGTGTAGCGCCTAT PA3135,
... ... PA3135 gltX PA3136 PA3137
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAACTGGTCTAT PA4902,
... ... PA4902 mdlC PA4900 PA4899 opdK
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGTTTATATGAT PA5029,
... ... PA5029 PA5028 PA5030
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAATTATTGATTTT PA5437,
... ... PA5437 PA5436 PA5435
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAGCAAGACTAT PA0717,
... ... PA0717 PA0718 PA0719 PA0720 PA0721 PA0722 coaB PA0724 PA0725 PA0726
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ATTTCTAACAACGAT PA0984
... ... PA0984
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
AACGGTTTTTATAAT aprA
... ... aprA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATATTTCCGACGAAT bdlA,
... ... bdlA PA1424
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGCTATCGCCGAT pvdG,
... ... pvdG pvdL pvdS
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAGACTCTCAAT ppx,
... ... ppx trxA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTTTTATTATTTAT PA0185,
... ... PA0185 PA0184 atsA PA0186
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTTTTATTATTTAT PA0186,
... ... PA0186 PA0185 PA0184 atsA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTAAAAACATTAAT pntAA,
... ... pntAA pntAB pntB
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - not specified not specified
ATAGATGCGGCCAAT PA0326,
... ... PA0326 PA0325 PA0324 PA0323 PA0327
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGTTATTGCCTAT alaS,
... ... alaS lysC
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGGAGAATCTAT aspS,
... ... aspS PA0962 pmpR ruvC ruvA ruvB PA0968 tolQ tolR tolA tolB oprL PA0974 PA0975 PA0976 PA0976.1
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - not specified not specified
ATATTTACCGGTTAT nadA,
... ... nadA mvfR
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGATTGCGATAAT PA1054,
... ... PA1054 PA1055 PA1056 PA1057 PA1058 PA1059 PA1060 PA1061 PA1062 PA1063
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAACGTCGGGTTAT cysP
... ... cysP
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGATTTTTTGTAT PA1507
... ... PA1507
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGATTGGCTTTTT PA1541,
... ... PA1541 PA1540
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ATTGGTCTGGTCTAT cynT,
... ... cynT cynS cynR
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGATATTTGCAAT PA2165,
... ... PA2165 PA2164 PA2163 PA2162 PA2161 PA2160 PA2159 PA2158 PA2157 PA2156 PA2155 PA2154 PA2166
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGAAGCACTTAAT rluC,
... ... rluC PA2974 PA2973 rne
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGACTCAGGCTAT snr1
... ... snr1
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
AAGTGTAGCGCCTAT PA3136,
... ... PA3136 PA3135 gltX PA3137
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGAAGCGCTCTAT PA3236,
... ... PA3236 PA3237 PA3238 PA3239 PA3240
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAAGTTCGGTGAAT mqoA,
... ... mqoA PA3451 PA3450
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
AATGAGAAAATCTAT PA3768,
... ... PA3768 PA3767
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATATAAAAACGCTAT rpsG,
... ... rpsG rpsL fusA1 tufA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGCTCCACTGATT rpsL,
... ... rpsL rpsG fusA1 tufA
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ACTGTTTTCAACAAT lpxC,
... ... lpxC PA4406.1
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ATCTCTATAATGAAT nadC,
... ... nadC PA4523
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTTCTTTTTACGAT mscL,
... ... mscL katB PA4612 PA4611
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAACTGGTCTAT mdlC,
... ... mdlC PA4900 PA4899 opdK PA4902
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGTTTATATGAT PA5030,
... ... PA5030 PA5029 PA5028
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGTTGATGCCGAT arcD,
... ... arcD arcA arcB arcC
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGTGATAGCTAAT PA5291
... ... PA5291
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGTTCTCTTTATT PA5370,
... ... PA5370 PA5371
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGAAAAGTTGAT PA0049,
... ... PA0049 PA0050
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGGAAAAGTTGAT PA0050,
... ... PA0050 PA0049
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
AATTATTTTTGTTAT tonB2,
... ... tonB2 exbB1 exbD1
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGATGCGGCCAAT PA0327,
... ... PA0327 PA0326 PA0325 PA0324 PA0323
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAAACTACGTAAAT PA0716,
... ... PA0716 PA0715
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGCCGCAGCCAAT PA0861,
... ... PA0861 PA0862
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCATCGGTAGTTAT PA0938
... ... PA0938
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
AGAGGCGGAGGCAAT PA0991,
... ... PA0991 PA0990
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTTGCCTTCTCGAT PA1198,
... ... PA1198 PA1199 PA1200
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGGCTTTATATAAT PA1383
... ... PA1383
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATATTTCCGACGAAT PA1424,
... ... PA1424 bdlA
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
AGCTCTTCAGACTAT PA1656,
... ... PA1656 PA1657 PA1658 PA1659
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATTGATAAAACCTAT PA2044
... ... PA2044
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGACCCATTCAAT PA2145,
... ... PA2145 PA2146 katE
Experimental technique details ChIP-chip (ECO:0006007) - Experimental technique details EMSA (ECO:0001807) - not specified not specified
ATTGATATTTGCAAT PA2166,
... ... PA2166 PA2165 PA2164 PA2163 PA2162 PA2161 PA2160 PA2159 PA2158 PA2157 PA2156 PA2155 PA2154
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGATTCAGTCTTT PA2228,
... ... PA2228 vqsM PA2226 PA2225 PA2229 PA2230
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGAGAGTACTAAT PA2229,
... ... PA2229 PA2228 vqsM PA2226 PA2225 PA2230
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGATTATTTGAAT PA2594
... ... PA2594
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGATCTGATCGAT PA2610,
... ... PA2610 PA2609
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCAAACGTATTTAT PA2935,
... ... PA2935 PA2936 PA2937
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCAAACGTATTTAT PA2936,
... ... PA2936 PA2935 PA2937
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATGGCTTTTTGCTAT PA3340
... ... PA3340
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAAAAAACATTTAT PA3446,
... ... PA3446 PA3445 PA3444 PA3443 PA3442 PA3441
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCTCTATAATGAAT PA4523,
... ... PA4523 nadC
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATCGACGTGATCTAT PA5180,
... ... PA5180 PA5179 PA5178 PA5181
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
TTTGAAAAAAACTAT PA5205,
... ... PA5205 argA
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGCAAAACTCTAT PA5546,
... ... PA5546 PA5547
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified
ATAGCAAAACTCTAT PA5547,
... ... PA5547 PA5546
Experimental technique details ChIP-chip (ECO:0006007) - not specified not specified