Curation Information

Publication
Autoregulation of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis.;Black DS, Irwin B, Moyed HS;Journal of bacteriology 1994 Jul; 176(13):4081-91 [8021189]
TF
HipB [P23873, view regulon], [P23874, view regulon]
Reported TF sp.
Escherichia coli BL21(DE3)
Reported site sp.
Escherichia coli BL21(DE3)
Created by
Erill Lab
Curation notes
-

Experimental Process

Deletion analysis coupled with beta-gal assays revealed that HipB self-represses, and that HipA has also a minor contribution to this effect. EMSA with fragments of the hipAB promoter region and HipB demonstrated binding to specific fragments. The region was then inspected with DNAse footprinting, identifying four inverted repeats.

The paper reports that TF forms complex with other proteins for binding with reported sites.

HipB forms complex with HipA, but the later is not strictly required for binding.

Transcription Factor Binding Sites


TATCCCCTTAAGCGGATA
TATCCTCACTAAAGGATA
TATCCCGTAGAGCGGATA
TATCCCCTTAAGCGGATA
TATCCTCACTAAAGGATA
TATCCCGTAGAGCGGATA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TATCCCCTTAAGCGGATA hipB,
... ... hipB hipA
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TATCCTCACTAAAGGATA hipB,
... ... hipB hipA
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TATCCCGTAGAGCGGATA hipB,
... ... hipB hipA
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer