Curation Information

Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence-specific and frame-dependent mRNA cleavage.;Prysak MH, Mozdzierz CJ, Cook AM, Zhu L, Zhang Y, Inouye M, Woychik NA;Molecular microbiology 2009 Mar; 71(5):1071-87 [19210620]
DinJ [Q47149, view regulon], [Q47150, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Erill Lab
Curation notes

Experimental Process

EMSA was used to prove that the DinJ-YafQ complex binds the palindrome upstream of the dinJ-yafQ operon.

The paper reports that TF forms complex with other proteins for binding with reported sites.

Manuscript reports only binding of complex DinJ-YafQ, not individual units.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... dinJ yafQ yafL
Experimental technique details EMSA (ECO:0001807) - repressor dimer