Curation Information

Publication
In vitro analysis of protein-operator interactions of the NikR and fur metal-responsive regulators of coregulated genes in Helicobacter pylori.;Delany I, Ieva R, Soragni A, Hilleringmann M, Rappuoli R, Scarlato V;Journal of bacteriology 2005 Nov; 187(22):7703-15 [16267295]
TF
Fur [B5Z6G7, view regulon]
Reported TF sp.
Helicobacter pylori G27
Reported site sp.
Helicobacter pylori G27
Created by
Matthew Coveyou
Curation notes
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Experimental Process

DNAse I footprinting identified two putative fur binding sites in the nikR-exbB intergenic region. DNAse I footprinting was also used to visualize competition assays between nikR and fur for these sites.

Transcription Factor Binding Sites


TAAATCCAGTTTGTATTATAATTGTTAATTTTA
TTGGATTTGATTGTAATTATTAGCTTAATCATTATT
TAAATCCAGTTTGTATTATAATTGTTAATTTTA
TTGAATTTGATTGTAATTATTAGCTTAATCATCATT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TAAATCCAGTTTGTATTATAATTGTTAATTTTA
... ... HPG27_1286 HPG27_1285 HPG27_1287 HPG27_1288 HPG27_1289
Experimental technique details DNAse footprinting (ECO:0005631) - not specified dimer
TTGAATTTGATTGTAATTATTAGCTTAATCATCATT
... ... HPG27_1286 HPG27_1285 HPG27_1287 HPG27_1288 HPG27_1289
Experimental technique details DNAse footprinting (ECO:0005631) - not specified dimer