Visual inspection of the ftsZ promoter identified a sequence with the perfect match to the CtrA consensus binding site. DNase I footprinting confirmed that CtrA binds to the predicted binding site. ftsZ-xylX fusion assays using ftsZ promoter in which CtrA binding site was deleted showed that ftsZ expression was 1.7-fold higher than in the wild-type strain.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|