Curation Information

A membrane-associated protein, FliX, is required for an early step in Caulobacter flagellar assembly.;Mohr CD, MacKichan JK, Shapiro L;Journal of bacteriology 1998 Apr; 180(8):2175-85 [9555902]
CtrA [P0CAW8, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes

Experimental Process

fliX-lacZ fusion in the C.crescentus strain showed that fliX expression was reduced by over 50% as a result of inactivation of ctrA. S1 nuclease protection assays determined fliX transcription start sites. Visual sequence inspection of the fliX promoter revealed a sequence with a seven out of nine bases match to the CtrA consensus sequence. DNase I footprinting determined that in the presence of CtrA, a single 17 bp region, partially overlapping the predicted binding motif, was protected.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... fliX flgI CCNA_02666 flbY
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Visual sequence inspection (nan) - activator not specified