fliX-lacZ fusion in the C.crescentus strain showed that fliX expression was reduced by over 50% as a result of inactivation of ctrA. S1 nuclease protection assays determined fliX transcription start sites. Visual sequence inspection of the fliX promoter revealed a sequence with a seven out of nine bases match to the CtrA consensus sequence. DNase I footprinting determined that in the presence of CtrA, a single 17 bp region, partially overlapping the predicted binding motif, was protected.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|