Curation Information

Publication
Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor.;Stephens C, Christen B, Watanabe K, Fuchs T, Jenal U;Journal of bacteriology 2007 Dec; 189(24):8828-34 [17933895]
TF
XylR [Q9A3Y5, view regulon]
Reported TF sp.
Caulobacter crescentus CB15
Reported site sp.
Caulobacter crescentus CB15
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

EMSA showed that XylR binds to the XylX promoter probe containing a XylR consensus sequence. xylX-lacZ fusion assays showed that xylX expression is repressed by XylR. Random mutagenesis of the xylX promoter verified the location of the XylR binding site.

Transcription Factor Binding Sites


TGTTAGCGCTACCA
TGTTAGCGCTACCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTTAGCGCTACCA CC_0823, CC_0822, CC_0821, CC_0820, CC_0819,
... ... CC_0823 CC_0822 CC_0821 CC_0820 CC_0819 CC_0818 CC_0817 CC_0816 CC_0824
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Random mutagenesis (ECO:0005530) - repressor not specified