DNA microarray analysis identified HnoC as a transcriptional regulator in Shewanella oneidensis, locating regulated hno-network signaling genes. GFP assays confirmed this regulation, characterizing HnoC as a repressor. EMSA demonstrated direct binding of HnoC to the hno promoter region. DNase I footprinting localized two binding sites. Multiple sequence alignment identified a consensus HnoC binding sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|