X-ray crystallography of the AmrZ:amrZ1 complex shows that AmrZ binds as dimer of dimers and reveals base specific contacts with the actGGCaaaacGCCggca sequence. Site directed mutagenesis to the GGC elements, coupled with fluorescence anisotropy was used to verify the importance of these contacts, as well as the AT-rich spacer. Site directed mutagenesis and fluorescence anisotropy were also used to interrogate the algD binding site ATTGGCCATTACCAGCCTCC.
Quantitative data format: Kd b (nM)GTACTGGCAAAACGCCGGCACG 8.41
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|