Eight binding sites identified in earlier publications were aligned according to binding affinity, four weak sites and four strong sites. Systematic mutagenesis of two representative binding sites was performed and binding affinity was determined by fluorescent anisotropy. It was shown that the presence of a thymine in the third position of the second pseudo-palindrome strongly influenced whether binding affinity was strong (thymine present) or weak (thymine absent).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|